Biojournal of Science and Technology
Volume 3, P-ISSN:2412-5377, E-ISSN:2410-9754, Article ID:m150006
Development of a modified Britton-Robinson buffer with improved linearity in the alkaline pH region
Akio Ebihara1, Shuhei Kawamoto2, Naoya Shibata2, Takashi Yamaguchi2, Fumiaki Suzuki1, Tsutomu Nakagawa1, *
1Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan
2Graduate School of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan
Date of Acceptance: Thursday, February 4, 2016
Date of Published: Saturday, February 20, 2016
Address corresponds to
Tsutomu Nakagawa
Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Email: nakagawa@gifu-u.ac.jp
Acedemic Editor: Editor-in-Chief
To cite this article
Akio Ebihara, Shuhei Kawamoto, Naoya Shibata, Takashi Yamaguchi, Fumiaki Suzuki, Tsutomu Nakagawa
.Development of a modified Britton-Robinson buffer with improved linearity in the alkaline pH region.Biojournal of Science and Technology.Volume 3,2016
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ABSTRACT
Britton-Robinson buffer is a multi-buffer system that consists of citric acid, phosphate, barbital, and boric acid. Although this buffer system is known to be effective from pH 2.6 to 12, the linearity of the alkaline region (pH 9–12) is poor. Searching for a weak acid additive to maintain the buffering capacity in the alkaline region, we found that glycine (pKa = 2.35 and 9.78) was effective in improving the linearity of the pH titration curve. The pH titration formula of the improved Britton-Robinson buffer is provided in this report. The pH-dependent activities of renin, a key enzyme that regulates blood pressure and electrolyte balance, were investigated using the improved buffer, and the results found were similar to those measured with the typical Britton-Robinson buffer.
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p-ISSN 2412-5377
e-ISSN 2410-9754
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