Biojournal of Science and Technology
Volume 1, ISSN:2410-9754, Article ID: m140006

Research Article

D4476, a cell-permeant inhibitor of CK1, potentiates the action of Bromodeoxyuridine in inducing senescence in HeLa cells

Sattya Narayan Talukdar¹, Dai Ayusawa², Md. Rasel Al Mahmud¹, Mohammad Nazir Hossain¹*

1. Department of Biochemistry, Primeasia University, Banani, Bangladesh

2 Department of Genome System Science, Graduate School of Nanobioscience, Yokohama City University, Japan

Date of Acceptance: Monday, December 29, 2014
Date of Published: Monday, January 26, 2015

Address corresponds to
Dr. Mohammad Nazir Hossain School of Science, Primeasia University, Banani, Bangladesh E-mail: nazir.hossain@primeasia.edu.bd

Acedemic Editor: Dr Shahdat Hossain

To cite this article
Sattya Narayan Talukdar, Dai Ayusawa, Md. Rasel Al Mahmud, Mohammad Nazir Hossain*.D4476, a cell-permeant inhibitor of CK1, potentiates the action of Bromodeoxyuridine in inducing senescence in HeLa cells.Biojournal of Science and Technology.Vol:1,2015

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ABSTRACT

To elucidate the mechanism of bromodeoxyuridine (BrdU) induced cellular senescence, we treated HeLa cells with D4476, a potent and specific inhibitor of casein kinase 1(CK1). We found that D4476 (10µM) treatment could arrest cell growth at G1 stage and induced cellular senescence when treated together with BrdU (10µM). However neither D4476 nor BrdU can induce cellular senescence alone, at a concentration of 10µM. These results suggest that the targets of CK1 may be involved in maintaining normal cellular process and their inactivation potentiates BrdU to induce senescence like phenomena in HeLa cells.